Table 2. Oligonucleotide sequences of the PCR and qRT-PCR primers.
Gene | Forward primer | Reverse primer |
---|---|---|
Lin28a | TTGTCTTCTACCCTGCCCTCT | GAACAAGGGATGGAGGGTTTT |
GAPDH | ATCACCATCTTCCAGGAGCGA | CCTTCTCCATGGTGGTGAAGAC |
Let-7a | GGTGAGGTAGTAGGTTGTATAGTT | Uni-miR qPCR primer (TaKaRa) |
U6 | ACGCAAATTCGTGAAGCGTT | Uni-miR qPCR primer (TaKaRa) |