Table 2. Pulse experiment with pCAME and LDN-193189.

Condition	Total n	Ectopic tails	Synergy Yes/No	Fisher’s Exact test P< 0.001
Control	90	0.0%	n/a
pCAME 70 µM	90	6.7%	n/a
LDN-193189 10 µM	90	0%	n/a
LDN-193189 5 µM	90	0%	n/a
pCAME 70 µM + LDN-193189 10 µM	90	35,6%	Yes	***
pCAME 70 µM + LDN-193189 5 µM	90	26.7%	Yes	***

Note: Embryos were pulsed for 1h at late gastrula/early somitogenesis (bud to 3-somite stages) and scored at 48 hpf; n/a: not applicable; Table represents data pooled from three independent experiments using (each time) 30 embryos per condition.

Fisher’s exact test: Pulse combinations vs. Control condition vs. pCAME condition vs. LDN-193189 conditions