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. 2013 Dec 11;8(12):e83510. doi: 10.1371/journal.pone.0083510

Figure 3. GAB2 does not confer nilotinib resistance in SUP-B15 cells.

Figure 3

(A) Expression of GAB2 and p-GAB2 proteins in JURL-MK2 and SUP-B15 cells was analyzed by Western blot. GAPDH levels served as a loading control. (B) JURL-MK2 and SUP-B15 cells were treated with different concentrations of nilotinib or BEZ235 as indicated for 6 h. P-GAB2, GAB2, p-CrkL and CrkL expression were determined by Western blot. (C) GAB2 was knocked down in JURL-MK2 and SUP-B15 cells by siRNA inhibition as described in Materials and Methods. After 24 h, expression of GAB2 was analyzed by Western blot and cell apoptosis was determined by annexin V/PI staining assay. Means ± SD of two experiments are shown. **P<0.01 vs control.