Skip to main content
. 2013 Dec 11;8(12):e83510. doi: 10.1371/journal.pone.0083510

Figure 5. Combining nilotinib and BEZ235 synergizes in inducing apoptosis in SUP-B15 cells.

Figure 5

(A) MDM2 knockdown in SUP-B15 cells was performed by siRNA, and MDM2 protein levels were examined by Western blot. SUP-B15 cells were treated with 200 nM nilotinib and/or MDM2 knockdown alone for 48 h. Apoptosis was analyzed by annexin V/PI staining assay. Means ± SD of three experiments are shown. *P<0.05 vs control. (B) SUP-B15 cells were treated with nilotinib (200 nM) and/or BEZ235 (2μM) for 24 h. Apoptotic cell death was determined by annexin V/PI staining assay. Means ± SD of three experiments are shown. **P<0.01 vs control. (C) SUP-B15 cells were treated with nilotinib (200 nM) or BEZ235 (2 μM) either alone or in combination for 24 h. Cell lysates were subjected to Western blot analysis with antibodies against caspase 3 and PARP as indicated. For equal protein loading GAPDH protein levels are shown.