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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Intravital. 2013 Aug 22;2(3):10.4161/intv.26138. doi: 10.4161/intv.26138

Figure 1.

Figure 1

Development of multicolored clones. (A) Multiple Rainbow cell lines were generated by transducing cells with LeGO vectors (Cerulean, Venus, and mCherry; see also Fig. 1). (B) Single clones from the MDA-MB-231 (shown here) and 4T1 Rainbow populations were then sorted and propagated. (C) Seven clones were selected by visual inspection based on color uniformity and intensity. (D) The seven clones were then analyzed using the described hue angle algorithm for their suitability for mixed population studies.