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Development of LeGO cancer cell lines. To generate Rainbow populations, multiple cell lines including SKBR3, MCF-7, MDA-MB-231, 4T1, Paca-2, and Panc02 were transduced at an approximate 50% infection rate with Cerulean, Venus, and mCherry LeGO vectors. The Rainbow populations were observed over three days to determine fluorescent protein stability (color or intensity changes).
