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Published in final edited form as: Biochim Biophys Acta. 2013 Sep 21;1840(1):10.1016/j.bbagen.2013.09.018. doi: 10.1016/j.bbagen.2013.09.018

Fig. 6 — Sulforaphane prevents ethanol-induced increases in lipid accumulation in HepG2 E47 cells. A: Levels of Nrf2 in E47 cells incubated with 6 uM sulforaphane or saline for 1,5 or 10 days. Results are expressed as the Nrf2/actin ratio and are shown under the blots. * P< 0.05 compared to saline, N=3 expreiments. B: Levels of TGs and C levels of cholesterol in E47 cells incubated with 100 mM ethanol or saline in the absence or presence of 6 uM sulforaphane for 7 days. * P< 0.05 compared to saline; ¥ P< 0.05 compared to ethanol alone. N=3 experiments. D: Oil Red O staining of E47 cells after 1 or 2 weeks of incubation with buffer alone, 100 mM ethanol alone, 6 uM sulforaphane alone or ethanol plus sulforaphane. Note the increased staining after 1 or 2 weeks of incubation with ethanol and the decrease in this staining when ethanol was incubated in the presence of sulforaphane.

Fig. 6 — Sulforaphane prevents ethanol-induced increases in lipid accumulation in HepG2 E47 cells. A: Levels of Nrf2 in E47 cells incubated with 6 uM sulforaphane or saline for 1,5 or 10 days. Results are expressed as the Nrf2/actin ratio and are shown under the blots. * P< 0.05 compared to saline, N=3 expreiments. B: Levels of TGs and C levels of cholesterol in E47 cells incubated with 100 mM ethanol or saline in the absence or presence of 6 uM sulforaphane for 7 days. * P< 0.05 compared to saline; ¥ P< 0.05 compared to ethanol alone. N=3 experiments. D: Oil Red O staining of E47 cells after 1 or 2 weeks of incubation with buffer alone, 100 mM ethanol alone, 6 uM sulforaphane alone or ethanol plus sulforaphane. Note the increased staining after 1 or 2 weeks of incubation with ethanol and the decrease in this staining when ethanol was incubated in the presence of sulforaphane.