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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Carcinog. 2013 Sep 9;53(11):907–916. doi: 10.1002/mc.22050

Figure 7.

Figure 7

The effect of Mcl-1 knockdown on WA-mediated apoptosis in MCF-7 cells. (A) Western blot analysis for Mcl-1 using lysates from SUM159 cells treated with the indicated doses of WA for specified time periods. The number on top of the immunoreactive band represents change of protein expression level relative to corresponding DMSO-treated control. (B) Western blotting for Mcl-1 and cleaved PARP using lysates from transiently transfected MCF-7 cells with a non-specific control siRNA or a Mcl-1-targeted siRNA and treated with 2.5 μM WA for 24 h. Quantitation relative to DMSO-treated control siRNA transfected cells is shown. (C) Histone-associated DNA fragment release into the cytosol in MCF-7 cells transiently transfected with a non-specific control siRNA or a Mcl-1 targeted siRNA and treated with 2.5 μM WA for 24 h. Quantitation relative to DMSO-treated control siRNA transfected cells is shown. Combined results (n = 6) from two independent experiments are shown as mean ± S.D. Statistical significance was determined by one-way ANOVA with Bonferroni’s multiple comparison test. aSignificantly different (P<0.05) compared with respective control. bSignificantly different (P<0.05) between groups at the same dose.