Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jun 26;65:10.1016/j.freeradbiomed.2013.06.034. doi: 10.1016/j.freeradbiomed.2013.06.034

Fig. 7.

Fig. 7

Knockout of Reg3β up-regulated transcription of Scly. (A) Binding frequencies of the four most frequent transcriptional factors in the promoter region (2000 base pair upstream of the transcription start site) of Scly. (B) qPCR analysis of hepatic mRNA levels for NF-1, YY1, c-jun, c-fos, JunB, FosB, Fra-1, Fra-2, and GR in the WT and KO mice that were normalized to the levels of Gapdh, n = 4–5/group. (C) Western blot analysis of hepatic c-Jun protein. (D) The ChIP assay of c-Jun binding to the AP-1 sites in the Scly promoter. The top panel shows the schematic overview of the AP-1 sites in the Scly promoter (the black box represents the AP-1 binding sites for each primer sets). The bottom left panel is a representative DNA gel image to show genotype differences in the binding of c-Jun protein to different AP-1 sites in the Scly promoter. The bottom right panel is a quantitative analysis of the ChIP assays based on three independent experiments.