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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Biochemistry. 2013 Aug 26;52(36):10.1021/bi400613h. doi: 10.1021/bi400613h

Figure 3. Oxidation and Cysteinylation destabilized SOD1.

Figure 3

Differential scanning fluorimetry was used to measure the melting temperature of SOD1, peroxide oxidized SOD1, and SOD-1 cys. Melting temperature of SOD1 (blue), peroxide oxidized SOD1 (red), and cysteinlyated SOD1 (green). Oxidation destabilized SOD1 by approximately 23 °C whereas cysteinylation destabilized SOD1 by approximately 5 °C. Folding-unfolding transitions (thermal denaturation curves) were measured in triplicate.