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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Biochemistry. 2013 Jul 22;52(31):10.1021/bi400779r. doi: 10.1021/bi400779r

Figure 4.

Figure 4

A) Sequence-selective cleavage of [5′-32P]-end labeled 64-nt hairpin DNA 7 by BLM A5. Lane 1, radiolabeled 7 alone; lane 2, 5 μM Fe2+; lane 3, 5 μM BLM A5; lane 4, 1 μM Fe(II)•BLM A5; lane 5, 2.5 μM Fe(II)•BLM A5; lane 6, 5 μM Fe(II)•BLM A5; lane 7, G+A lane. B) Sequence-selective cleavage of [3′-32P]-end labeled 64-nt hairpin DNA 7 by BLM A5. Lane 1, radiolabeled 7 alone; lane 2, 10 μM Fe2+; lane 3, 5 μM BLM A5; lane 4, 1 μM Fe(II)•BLM A5; lane 5, 2.5 μM Fe(II)•BLM A5; lane 6, 5 μM Fe(II)•BLM A5; lane 7, Maxam-Gilbert G+A lane.