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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Biochemistry. 2013 Aug 29;52(36):10.1021/bi4008353. doi: 10.1021/bi4008353

Figure 4.

Figure 4

Aggregates of human γD-crystallin. TEM images of undamaged (A) and UV-B photodamaged (B) γD-crystallin (S84C) show the presence of spherical bodies in both samples, and the formation of fibers in the photodamaged sample after 6 hours of illumination. Analysis of the diameters of spheres and fibers in both samples (C-E) shows a slight increase in sphere sizes from ∼20 nm to ∼30 nm and the formation of fibers with a mean diameter of ∼6 nm. The aggregation of wild type (open circles) and S84C (closed circles) γD-crystallin is accompanied by an increase in ThT fluorescence over the course of 9 hours (F).