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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jul 11;65:10.1016/j.freeradbiomed.2013.07.005. doi: 10.1016/j.freeradbiomed.2013.07.005

Fig. 3. Validation of the specificity of the Nox5 monoclonal antibody.

Fig. 3

UACC-257 cells were stably transfected with either the vector pcDNA 3.1 or pcDNA3-HA-Nox5β plasmid and selected with G418. Several clones were isolated, of which two vector clones (1 and 2) and two Nox5-overexpressing clones (1 and 2) were used for this study. Nox5 overexpression was confirmed both at the mRNA (A) and protein (B) levels. The HA tag expression confirmed the specificity of the Nox5 mouse monoclonal antibody for Nox5 overexpression. C and D, Transient knockdown of Nox5 expression in the stably Nox5 overexpressing clone 2 with either scrambled control or two different Nox5-specific siRNAs. The decrease in Nox5 expression was confirmed at the protein (C) and mRNA (D) levels. **, p < 0.01