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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jul 11;65:10.1016/j.freeradbiomed.2013.07.005. doi: 10.1016/j.freeradbiomed.2013.07.005

Fig. 4. Evaluation of cross-reactivity of the mouse monoclonal antibody to NADPH homologues of Nox5.

Fig. 4

RNA (A) and Western blot (B) analysis was carried out using samples positive for Nox1 (HT-29 colon cancer cell line), Nox2 (HEK-293 cells transiently transfected with DDK-tagged Nox2 plasmid), Nox4 (HEK-293 cells transiently transfected with Myc-tagged Nox4 plasmid), Nox5 (UACC-257 parental cells and Nox5 overexpressing cells), and Duox (BxPC-3 pancreatic cells treated with 25 ng/ml IFN-γ for 24 h) expression. Antibodies used to detect the various NADPH homologues are detailed in the Protocol section.