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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jul 11;65:10.1016/j.freeradbiomed.2013.07.005. doi: 10.1016/j.freeradbiomed.2013.07.005

Fig. 6. Flow cytometric detection of Nox5 in UACC-257-Nox5 overexpressing cells.

Fig. 6

Log-phase UACC-257 cells that stably express Nox5 (Clone 2) or the vector control (Clone 1) were fixed, permeabilized, and labeled with 5 µg of purified Nox5 mouse monoclonal antibody as described in the Protocol section. Cells labeled with the antibody were staine d with Alexa Fluor 488 goat anti-mouse antibody (1:1000), and the fluorescence was detected by flow cytometry. Unstained cells and cells labeled with irrelevant mouse monoclonal IgG antibody (5 µg) represent background staining controls. Images are representative of at least 3 experiments.