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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2013 Jul 18;65:10.1016/j.freeradbiomed.2013.07.019. doi: 10.1016/j.freeradbiomed.2013.07.019

Figure 1.

Figure 1

Representative 2D gels used for proteomics identification of differentially expressed proteins. Brain samples of rats fed a control-, low- or high-VitD diet from middle-age to old-age were separated by IEF using IPG strips pH 3–10 followed by SDS-PAGE using 8–16% Tris-HCl gels. Separated proteins were visualized with SYPRO Ruby protein gel stain. Acquired images were analyzed using PDQuest software and spots showing different intensities were chosen for further analysis. The figure indicates a comparison of the PrxIII spot as a representative of those found with significant expression differences in low vs. control VitD groups as identified by PDQuest analysis. Below are expansions of the spot images showing clearly that the PrxIII level is elevated in brain of rats on a chronic low VitD diet.