Fig. 5.

Role of NOS uncoupling on insulin resistance-induced Ca²⁺ leak. Cardiomyocytes from control or sucrose-fed mice with or without folic acid treatment were assessed for intracellular Ca²⁺ levels for 80 seconds in the presence of a 0Na⁺-0Ca²⁺ buffer. A: Representative traces from control and insulin resistant murine cardiomyocytes with and without the RYR inhibitor tetracaine (1 mM); B: Diastolic Ca²⁺ leak as calculated by the difference in AUC between 0Na⁺-0Ca²⁺ and 0Na⁺-0Ca²⁺+tetracaine; C: Number of spontaneous contraction. Mean ± SEM, n = 18 – 30 cells from 3 mice per group, *p < 0.05 vs. Control, #p < 0.05 vs. Sucrose group.