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. Author manuscript; available in PMC: 2013 Dec 12.
Published in final edited form as: J Genet Syndr Gene Ther. 2013 Jun 17;4(5):1000147. doi: 10.4172/2157-7412.1000147

Figure 2. In all conditions tested aortas from females gave a reduced angiogenic response compared to males.

Figure 2

(A) VEGF promotes angiogenesis in rat aortas embedded in collagen I in serum free conditions. Age matched Male and female rat aortic rings were seeded as described and supplemented with the concentrations of VEGF indicated.

(B) High level of FBS can be inhibitory to the angiogenic response from both male and female rat aortas. Rings were embedded in collagen I and supplemented with 10ng/ml VEGF and the concentrations (v/v) of FBS indicated.

(C) The formation of angiogenic sprouts from murine aortas requires VEGF.

(D) High levels of serum also inhibit angiogenic sprout formation from murine vessels.

Murine aortic explants were supplemented with 30ng/ml VEGF and the concentrations of FBS indicated. In (A-D) angiogenic sprouts were counted 4 days (rat) and 8 days (mouse) post embedding. At least 10 rings/animal, were counted and the mean calculated, data presented for each condition represents the mean of mean sprout formation obtained from at least 3 different animals (n-numbers for each condition are shown above data bars). Error bars represent the standard error of the mean and significance between male and female samples at each condition was calculated using a 2-way ANNOVA comparison.