Table 1. Oligonucleotide sequences and qRT–PCR conditions.
Gene | Primer sequences (fwd/rev) | Ta (°C) | Tm (°C) |
---|---|---|---|
β-Actin |
5′-CTA CGT CGC CCT GGA CTT CGA GC-3′
5′-GAT GGA GCC GCC GAT CCA CAC GG-3′ |
60–68 |
80 |
AGO2 |
5′-GTC TCT GAA GGC CAG TTC CA-3′
5′-ATA GAG GCC TCA CGG ATG G-3′ |
60 |
78 |
cJun |
5′-TTC CTC CCG TCC GAG AGC GG-3′
5′-TCG GCG TGG TGG TGA TGT GC-3′ |
60 |
74 |
NRAS | 5′-ATG AGG ACA GGC GAA GGC T-3′ 5′-TGA GTC CCA TCA TCA CTG CTG-3′ | 60 | 66 |