Figure 5.

Inhibition of caspase-4 reduces persin-induced apoptosis. (A) MCF-7 and T-47D cells were treated with an apoptotic concentration of persin (10 μg ml⁻¹) alone or in combination with caspase-4 inhibitor (20 μM) or pan-caspase inhibitor (20 μM) for 24 h, and then attached and floating populations were analysed for M30-FITC positivity by flow cytometry. Representative histograms showing the M30-FITC-positive (apoptotic) fraction are shown. (B) Bars, mean of M30-positive fractions from three independent experiments±s.e. ***P<0.001; ****P<0.0001 for combination treatment vs single treatment.