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RT-PCR product analysis on agarose gel. Ten microliters of the PCR product was electrophoresed on 3% agarose gels containing ethidium bromide. L, a 1 kb plus molecular DNA ladder (Invitrogen). H, human colon cell line HCT-116. 2 to 15, patient ID numbers. A. Expression of the second primer pair products (234 bp) with the 10 liquid nitrogen preserved samples. B. Expression of the third primer pair products (103 bp).
