Table 1. PCR primer sets and expected amplicon sizes tested for the β-actin gene.
Primer sets | Amplicon size | |
---|---|---|
(1) | Forward 5′-ACACTGTGCCCATCTACGAGG-3′ | 620 bp |
Reverse 5′-AGGGGCCGGACTCGTCATACT-3′ | ||
(2) | Forward 5′-GGACTTCGAGCAAGAGATGG-3′ | 234 bp |
Reverse, 5′-AGCACTGTGTTGGCGTACAG-3′ | ||
(3) | Forward 5′-CCCAGCACAATGAAGATCAA-3′ | 103 bp |
Reverse 5′-ACATCTGCTGGAAGGTGGAC-3 |