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. 2013 Dec 15;126(24):5635–5644. doi: 10.1242/jcs.132795

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© 2013. Published by The Company of Biologists Ltd

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Fig. 1. — K8 and K18 are main keratins in the endocrine pancreas of K8^+/+ mice but low levels of K7 also exist, allowing marginal keratin filament formation in K8^−/− mice. (A) Immunostaining of pancreatic sections from K8^+/+ and K8^−/− mice for insulin (red), K8, K18 or K19 (green) and nuclei (blue). K8 and K18 are the most prominent keratins in the islets and some K19-positive cells are seen in K8^+/+ islets. The white arrow in the upper panel points to K19-positive cells and the inset shows a higher magnification of the K19-positive and insulin-negative cells that form occasional duct-like structures within the islet in K8^+/+ mice. K8^−/− mice lack K8 but express K18 in islets and K19 in exocrine ducts (indicated by the white arrow in the lower panel). Scale bar: 100 µm. (B) Islet cells in pancreatic sections from K8^+/+ and K8^−/− mice stained for K7 (green), K18 (red) and nuclei (blue). Arrows show colocalisation of K7 and K18. Scale bar: 10 µm. (C) Western blot for K8, K18 and Hsc70 of islet total lysates (lane 1, K8^+/+; lane 2, K8^−/−), for K8 and K18 of HSE islet lysate (lane 3, K8^+/+; lane 4, K8^−/−) and liver HSE (lane 5) used as positive control. Lane 6 shows a shorter exposure of the K8 and K18 signals seen in lane 5.