Fig. 2.

Decreased total Ir expression in tumors from Apc^Min/+ mice is associated with reduced IR-B expression and altered levels of other insulin–IGF axis mRNAs. qRT-PCR measured (A) total Ir, (C) Igf1, (D) Igf1r, (E) Irs2 and (F) Irs1 mRNA in small intestine and colonic tumors from Apc^Min/+ mice compared with normal small intestine and colon tissue from wild-type animals (WT). (B) RT-PCR was carried out to measure the ratio of IR-B∶IR-A mRNA expression using primers spanning exon 11 of the Ir in small intestine and colon tumor tissue compared with normal tissue. All qRT-PCR data are normalized to ribosomal protein S6, invariant control. Data represent mean ± s.e.m. (n≧5); *P≤0.05 in Apc^Min/+ tumor versus WT normal tissue; unpaired t-test.