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. 2013 Dec 11;54(13):8051–8062. doi: 10.1167/iovs.13-12844

Figure 1.

Figure 1

The PicoGreen dye assay to measure DNA abundance. (A) Fluorescence signal intensity measurement of λDNA with the PicoGreen dye (1:200 dilution) shows a linear relationship (r = 0.996). (B) The PicoGreen dye kinetic assay of λDNA and tear fluid from a patient having DED with and without DNase I addition. Stable emission of fluorescence is observed from the λDNA-PicoGreen dye complex. The addition of exogenous DNase I to the λDNA-PicoGreen dye complex leads to an exponential decay in fluorescence signal due to DNA degradation. Similarly, on the addition of DNase I to tear fluid eDNA-PicoGreen dye complex, fluorescence signal intensity decreases exponentially over time, indicating that the eDNA present in tear fluid is degraded by DNase I. RFU, relative fluorescence units.