Table 1.
Binding constants (M−1) obtained from SPR experiments for all compounds and AATT, TTAA and CG hairpins. The corresponding ΔTm (°C) values in poly(dA)·poly(dT)14 are shown for comparison
| Compd. | X | ΔTm (°C)a poly(dA)·poly(dT) | Kaatt ×105 (M−1) | Kttaa ×105 (M−1) | Kcg ×105 (M−1) |
|---|---|---|---|---|---|
| 1 | CH2 | 17.2 | 0.49 | 0.50 | ≪1 |
| 2 | CH2CH2 | 29.0 | 2.90 | 0.75 | ≪1 |
| 3 | O | 25.3 | 4.70 | 0.67 | ≪1 |
| 4 | S | 23.0 | 1.20 | 2.40 | 1.80 |
| 5 | NH | 35.1 | 6.40 | 2.40 | ≪1 |
| 6 | Piperazine | 31.1 | 2.00 | 0.80 | ≪1 |
| 7 | CO | 32.0 | 2.70 | 4.60 | 3.60 |
| 8 | NHCONH | 30.0 | 1.30 | 0.65 | ≪1 |
a
Bp/D = 3. Poly(dA)·poly(dT) melting temperature in MES buffer (10 mM) is 43 °C without added NaCl. The binding constants are the primary binding constants (K1) and were collected with 92 mM NaCl.