Skip to main content
. Author manuscript; available in PMC: 2013 Dec 12.
Published in final edited form as: Org Biomol Chem. 2010 Oct 15;8(24):10.1039/c0ob00428f. doi: 10.1039/c0ob00428f

Table 1.

Binding constants (M−1) obtained from SPR experiments for all compounds and AATT, TTAA and CG hairpins. The corresponding ΔTm (°C) values in poly(dA)·poly(dT)14 are shown for comparison

Compd. X ΔTm (°C)a poly(dA)·poly(dT) Kaatt ×105 (M−1) Kttaa ×105 (M−1) Kcg ×105 (M−1)
1 CH2 17.2 0.49 0.50 ≪1
2 CH2CH2 29.0 2.90 0.75 ≪1
3 O 25.3 4.70 0.67 ≪1
4 S 23.0 1.20 2.40 1.80
5 NH 35.1 6.40 2.40 ≪1
6 Piperazine 31.1 2.00 0.80 ≪1
7 CO 32.0 2.70 4.60 3.60
8 NHCONH 30.0 1.30 0.65 ≪1
a

Bp/D = 3. Poly(dA)·poly(dT) melting temperature in MES buffer (10 mM) is 43 °C without added NaCl. The binding constants are the primary binding constants (K1) and were collected with 92 mM NaCl.