Table 2.
Binding constants (×105,M−1) calculated using Scatchard plot analysis from the UV titrations, CD and ITC experiments performed with compounds 5 and 7 with both natural DNA and poly(dA·dT)2, using phosphate buffer. The corresponding binding constants (×105,M−1) obtained with SPR in the AATT hairpin are also included for comparison
| AATT |
Natural DNA |
poly(dA·dT)2 |
||||
|---|---|---|---|---|---|---|
| SPR | UV-titrat. | ITC | UV-titrat. | CD | ITC | |
| 5 | 6.4 | 0.3 ±0.1 | 0.3 ±0.1 | 2.5 ±0.3 | 1.2 ±0.7 | 0.6 ±0.1 |
| 7 | 2.7 | – | 0.9 ±0.3 | 0.6 ±0.1 | 0.3 ±0.1 | 0.6 ±0.1 |