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. Author manuscript; available in PMC: 2013 Dec 12.
Published in final edited form as: Oncogene. 2001 Feb 1;20(5):10.1038/sj.onc.1204133. doi: 10.1038/sj.onc.1204133

Fig. 3. PDGF-C is a more potent transforming agent than EWS/FLI.

Fig. 3

Polyclonal NIH 3T3 cellular populations stably expressing either PDGF-C, EWS/FLI or vector alone (neo) were developed and assayed for transformation both (a) in vitro and (b) in vivo. (a). Specific quantities of cells expressing each construct were plated in soft agar as described in Methods. The EWS/FLI and neo plates shown are at a 10-fold greater cell density than that of the PDGF-C plate. As can be seen, PDGF-C expression induces a much greater degree of anchorage-independent growth than does EWS/FLI or vector alone. This experiment was performed three times, and a typical result is shown. (b). 1 × 106 cells expressing each construct were injected into nude mice. After two weeks, the average resulting tumor mass was markedly higher in mice injected with PDGF-C-expressing cells (2.81 grams) than in those injected with cells expressing EWS/FLI (0.07 grams). Cells expressing vector alone resulted in no detectable tumor formation at this time point (data not shown).