Table 1. Effect of Vav1 Mutations on SLP-76 Microcluster Dynamics.
SLP-76 microcluster dynamics were measured in JV.SC cells transfected with vectors expressing either mYFP or the indicated Vav1.mYFP chimeras. Individual SLP-76 microcluster paths were obtained by manual tracing, and average microcluster behaviors were calculated per cell. The values presented are the means of values obtained in the indicated number of cells.
| Persistence (sec) | p. | Movement (μm) | p. | Speed (nm/sec) | p. | % SLP in Clusters | p. | Clusters per cell | p. | N (Cells) | N (Expts) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mYFP | 68.5 ± 3.92 | †† | 0.6 ± 0.03 | †† | 56.6 ± 2.12 | †† | 5.9 ± 0.66 | †† | 10.8 ± 0.83 | †† | 45 | 13 |
| WT | 180.5 ± 6.94 | ** | 3.3 ± 0.19 | ** | 102.9 ± 3.25 | ** | 43.5 ± 2.83 | ** | 19.1 ± 0.8 | ** | 46 | 13 |
| N-SH3* | 164.9 ± 11.6 | ** | 2.6 ± 0.26 | ** | 99.6 ± 4.79 | ** | 33.9 ± 3.68 | ** | 20.6 ± 1.51 | ** | 15 | 3 |
| C-SH3* | 181.6 ± 10.94 | ** | 3.1 ± 0.28 | ** | 104.8 ± 4.54 | ** | 40.8 ± 4.44 | ** | 22.6 ± 1.17 | **,† | 15 | 3 |
| SH2* | 74.2 ± 8.5 | †† | 0.6 ± 0.05 | †† | 52.7 ± 4.35 | †† | 7.4 ± 1.34 | †† | 13.6 ± 1.81 | †† | 15 | 3 |
| N3*C3* | 98.3 ± 11.83 | **,†† | 1.9 ± 0.33 | **,†† | 85.4 ± 8.04 | **,† | 24.5 ± 5.98 | **,† | 16 ± 1.86 | * | 10 | 3 |
| Δ323 | 62.1 ± 5.11 | †† | 0.7 ± 0.07 | †† | 55.5 ± 5.04 | †† | 6.5 ± 1.29 | †† | 12.4 ± 1.97 | †† | 10 | 3 |
| SH323 | 91.4 ± 12.91 | *,†† | 0.8 ± 0.1 | †† | 70.8 ± 8.75 | *,†† | 11.2 ± 3.4 | *,†† | 14.2 ± 1.65 | † | 10 | 3 |
| Y174F | 165.6 ± 10.14 | ** | 3 ± 0.3 | ** | 98.1 ± 5.07 | ** | 25.6 ± 2.75 | **,†† | 15.7 ± 0.95 | **,† | 15 | 3 |
| Y3F | 158.4 ± 15.11 | ** | 2.9 ± 0.41 | ** | 99.4 ± 8.65 | ** | 22.1 ± 3.34 | **,†† | 13.6 ± 0.98 | †† | 11 | 3 |
| L213A | 101.6 ± 11.99 | **,†† | 0.9 ± 0.1 | *,†† | 66.4 ± 3.64 | *,†† | 12.2 ± 2.3 | **,†† | 15.1 ± 1.83 | †† | 12 | 3 |
| L278Q | 93.9 ± 15.04 | *,†† | 1.4 ± 0.3 | **,†† | 73.3 ± 8.8 | *,†† | 11.6 ± 4.45 | *,†† | 10.5 ± 1.38 | †† | 11 | 3 |
| LK-AA | 170.8 ± 7.34 | ** | 2.9 ± 0.31 | ** | 101.2 ± 5.13 | ** | 32.2 ± 3.26 | **,† | 18.8 ± 1.44 | ** | 15 | 3 |
| ΔCH | 153 ± 10.09 | **,† | 2.1 ± 0.22 | **,†† | 90.2 ± 3.84 | **,† | 23.6 ± 3.49 | **,†† | 20.6 ± 1.4 | ** | 15 | 3 |
| ΔDH-PH | 166.6 ± 13.41 | ** | 2.2 ± 0.33 | **,† | 82.3 ± 4.72 | **,† | 28.9 ± 3.57 | **,† | 17.4 ± 1.66 | ** | 10 | 3 |
Significant differences from the control JV.SC cells transfected with an mYFP expression vector are indicated with asterisks:
*
for p < 0.05,
**
for p < 0.005.
Significant differences from JV.SC cells reconstituted with wild-type Vav1.mYFP are indicated with daggers:
†
for p<0.05,
††
for p<0.005.