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. 2013 Nov 6;7(1):17–22. doi: 10.3892/etm.2013.1382

Figure 2.

Figure 2

(A) Immunosuppressive effects of ASCs on alloreactively stimulated spleen cells. In each well, the indicated numbers of ASCs were mixed with 4×105 spleen cells and 4×105 CD3/CD28 beads. (B) Indirect (a) and direct (b) effects of ASCs on anti-CD3/CD28 bead-stimulated spleen cells were examined using Transwell chambers. (a) Spleen cells (4×105) were seeded with anti-CD3/CD28 beads in the upper chamber in the presence (right column) or absence (left column) of 1.6×105 ASCs seeded in the lower chamber. (b) The same number of spleen cells were seeded with anti-CD3/CD28 beads in the presence (right column) or absence (left column) of ASCs in the lower chambers. Production of IFN-γ in the supernatant (ng/ml) is indicated by the ordinate. Experiments were repeated in triplicates and results are described as the means ± standard deviation. The Student’s t-test was used to test the probability of significant differences between samples. *P<0.05 and **P<0.01. ASCs, adipose tissue-derived mesenchymal stromal cells; IFN-γ, interferon-γ.