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. 2013 Nov 6;7(1):17–22. doi: 10.3892/etm.2013.1382

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Flow cytometric analysis of T-cell subsets in CD45⁺ spleen cells. Spleen cells (4×10⁵) were cultured in the presence or absence of 4×10⁵ anti-CD3/CD28 beads and ASCs for 12 h. The cells were harvested, then FACS analyses were performed using anti-CD45 (PerCP-Cy™5.5), anti-CD4 (PE), anti-CD8 (FITC) and anti-CD69 (APC) antibodies. The percentage of each cell subpopulation (denoted on the right side) among the CD45⁺ total lymphocytes are indicated. ASCs, adipose tissue-derived mesenchymal stromal cells.