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. 2013 Nov 6;7(1):17–22. doi: 10.3892/etm.2013.1382

Figure 4.

Figure 4

(A) Suppression of endogenous β2M transcripts in ASCs transfected with β2M-specific siRNA. The expression of endogenous β2M mRNA in siRNA-transfected ASCs was confirmed by semi-quantitative PCR. The relative expression level of β2M mRNA was normalized as a ratio to that of RPS5-mRNA. Results are described as a mean of duplicate experiments. (B) IFN-γ production by stimulated spleen cells in the presence or absence of siRNA-transfected ASCs. Anti-CD3/CD28-stimulated spleen cells were directly (a), or indirectly (b) co-cultured with β2M-specific or control (scrambled) siRNA-transfected ASCs. Relative IFN-γ levels in the supernatant are indicated by the ordinate. Experiments were repeated twice with good reproducibility. Results from a representative experiment are summarized. Values in more than triplicates were described as the mean ± standard deviation. The Student’s t-test was used to test the probability of significant differences between samples. #P<0.05 β2M siRNA vs. scrambled siRNA; **P<0.01 β2M siRNA or scrambled siRNA vs. control (without ASCs). β2M, β2 microglobulin; ASCs, adipose tissue-derived mesenchymal stromal cells; RPS5, ribosomal protein S5; IFN-γ, interferon-γ.