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. Author manuscript; available in PMC: 2013 Dec 12.
Published in final edited form as: Dev Biol. 2013 Aug 29;383(1):90–105. doi: 10.1016/j.ydbio.2013.08.015

Fig. 6.

Fig. 6

Altered SHH and FGF signaling causes polydactyly and syndactyly. Shh domain was expanded along the anterior–posterior and proximal–distal axis in Sost–/– and Sost–/–; Sostdc1–/– relative to WT and Sostdc1–/– limb buds at E10.5 (A, arrows, bracket) and E1 1.5 (D). Downstream of Shh, Gli1 expression was dramatically expanded in Sost–/–; Sostdc1–/– E11.5 limb buds relative to all other genotypes (E, brackets). Grem1 expression was absent in Shh positive region, and in Sostdc1–/– and Sost–/–; Sostdc1–/– limbs the Grem1 domain was reduced on the posterior side (B, brackets). Fgf8 AER expression domain was expanded and disorganized in Sost–/– and Sost–/–; Sostdc1–/– limbs, and on the anterior side of the Sost–/–; Sostdc1–/– limbs Fgf8 expression was reduced in all time points examined (B and F–G, arrows). A reduction in interdigital apoptosis was also detected on the anterior side of Sost–/–; Sostdc1–/– limb buds at E12.5 (I, arrow).