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. 2013 Dec 12;9(12):e1004025. doi: 10.1371/journal.pgen.1004025

Figure 2. Ethylene signalling positively promotes hypocotyl growth and HY5 degradation.

Figure 2

(A) Morphological observations and (B) statistical analyses of hypocotyl length after 5 days of incubation in MS medium supplemented with or without 10 µM ACC. The data indicate the mean values plus the SD from three independent experiments with approximately 30 seedlings. P-values (ACC treatment vs. non-treatment) were determined with a two-tailed Student's t-test assuming equal variances (*P<0.05). (C) HY5 accumulation after treatment with or without ACC treatment. Protein extracts were prepared from 5-day-old seedlings after treatment with or without 25 µM ACC for 15 h. Immunoblotting was performed with anti-HY5 and -TUB4 antibodies. The TUB4 signals indicate equal protein loading. Numbers indicate the relative protein levels of HY5.