(A) One hundred males of each wMel variant line and w1118 iso were pricked with FHV (109 TCID50/ml) and survival was followed daily. One more replicate was performed with similar results. (B) Cox hazard ratio of each wMel variant line compared to w1118 iso when infected with FHV (109 TCID50/ml). The natural logarithm of the Cox hazard ratio is shown. Error bars represent standard error. Letters refer to compact letter display of Tukey's test of all pairwise comparisons. Analysis is based on two independent replicates, one with 100 flies per line and one with 50 flies per line, with 10 flies per vial. w1118 iso is assigned to group “d” in the compact letter display of Tukey's test (not shown). (C) One hundred males of each tetracycline treated line, derived from the wMel variants lines and w1118 iso, were pricked with FHV (108 TCID50/ml) and survival was followed daily. (D) Cox hazard ratio of each tetracycline treated line, derived from the wMel variants lines, compared to w1118 iso tetracycline treated line, when infected with FHV (108 TCID50/ml). Analysis is based on one replicate with 100 flies per line, 10 flies per vial. w1118 iso tetracycline treated line is assigned to group “a” in the compact letter display of Tukey's test (not shown). (E) 3–6 day old males of each wMel variant line and w1118 iso were pricked with FHV (109 TCID50/ml) and collected 3 days later for RNA extraction and RT-qPCR. Relative amount of FHV was calculated using host Rpl32 mRNA as a reference and values are relative to median of wMelCS_b samples. Each point represents a replicate (ten males per replicate, four replicates per Drosophila line), and lines are medians of the replicates. FHV loads are not significantly different between wMel and wMelCS-like variants (linear mixed-effect model, p = 0.5347).