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. 2013 Dec 12;9(12):e1003896. doi: 10.1371/journal.pgen.1003896

Figure 7. Genomic region amplified in wMelPop.

Figure 7

(A) Depth of coverage of sequence reads of wMelPop mapped to wMel reference genome (GenBank: AE017196) in region 484,564 to 512,000. Nucleotide positions, predicted genes, RT repeats and the ISWpi1 element in this region of wMel are shown. The 5′ RT repeat extends from 486,532 to 488,449 (1912 bp). The 3′ RT repeat in wMel extends from 507,470 to 510,325 but is split in two parts due to the insertion of an ISWpi1 (IS5) transposon from 507,928 to 508,848. This ISWpi1 is not present in wMelPop or the closely related wMelCS_b. Figure modified from USCS genome browser (http://genome.ucsc.edu/) [121], [122]. (B) Relative amounts of genomic copy number of Octomom genes (WD0506–14), genes adjacent to Octomom region (WD0505 and WD0519) and control gene rpoD in wMel, wMelCS_a, wMelCS_b and wMelPop were calculated using wsp as a reference gene. Values are relative to median of wMelCS_b samples. Each point represents a replicate (ten males per replicate, three replicates per Drosophila line) and lines are medians of the replicates.