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. 2013 Mar;65(2):158–167. doi: 10.1016/j.ihj.2013.02.012

Fig. 2.

Fig. 2

The restriction digestion pattern of CYP2C19*2 and CYP2C19*3 on 10% polyacrylamide gel electrophoresis. Lane 1: PCR products of 321 bp and 271 bp for CYP2C19*2 and CYP2C19*3 respectively. Lane 2–Lane 4: wild type genotype (*1 × *1) of CYP2C19*2 showing two bands 212 bp and 109 bp and wild type genotype (*1 × *1) of CYP2C19*3 showing two bands 175 bp and 196 bp. Lane 3: Heterozygous genotype (*1 × *2) of CYP2C19*2 showing three bands 321 bp, 212 bp and 109 bp  and wild type genotype (*1 × *1) of CYP2C19*3 showing two bands 175 bp and 196 bp. Lane 4: homozygous mutant genotype (*2 × *2) showing one band of 321 bp and wild type genotype (*1 × *1) of CYP2C19*3 showing two bands 175 bp and 196 bp. Lane 5: O′ Range Ruler, 20 bp, ready to use DNA Ladder from MBI Fermentas.