Figure 1. The production of IFN-γ and TNF-α by both CD8⁺ and CD4⁺ T cells from pleural fluid cells (PFCs) by patients with tuberculous pleurisy (TBP) in response to 13-15aa culture filtrate protein 10 (CFP10) peptides.

(A, B) PFCs from patients with TBP were cultured with or without a mixture of twenty-six CFP10 peptides for 8 h and stained with anti-CD4, CD8, IFN-γ and TNF-α mAbs. The expression of IFN-γ and TNF-α by CD8⁺ (A) and CD4⁺ (B) T cells was analyzed. Numbers in quadrants indicate percentages of the cells in each population. (C, D) Statistical results of IFN-γ and TNF-α expression by CD8⁺ (C) and CD4⁺ (D) T cells from twenty-seven patients with TBP. Open circles indicate cultures without CFP-10 and filled circles indicate cultures with CFP-10 peptides. Horizontal bars indicate the medians for the indicated conditions. ***, P<0.001. Dashed line represents the cutoff of IFN-γ or TNF-α-positive CD8⁺ or CD4⁺ T cells.