. 2013 Aug 9;69(1):12–20. doi: 10.1093/jac/dkt316

Table 3.

Phenotypes and genotypes of isolates with G190E and a site-directed mutant with G190G

Isolate^a	Reference	Fold resistance				Mutations^b
Isolate^a	Reference	NVP	EFV	ETR	RPV	Mutations^b
21 176	Stanford University	200	200	200	200	V60I, D67G, S68G, K70R, L74V, I135V, E138A, K173E, I178M, G190E, R211A, K219N, V245E, E248D, D250E, A272P, T286A
21176G190^E→G		0.7	1.2	2.3	2.0	V60I, D67G, S68G, K70R, L74V, I135V, E138A, K173E, I178M, R211A, K219N, V245E, E248D, D250E, A272P, T286A
24 746	Stanford University	200	200	94	28	K20R, D67N, K70R, K122E, I135V, E138A, S162C, M184MV, G190E, T200A, F214M, T215F, K219Q, L228H, V245E
59 209	Stanford University	200	200	110	7.3	V35L, D67N, K70R, K101R, K103R, D177E, I178M, G190E, G196E, T200A, E204N, R211K, T215F, K219E, L228H, V245E, A272P, T286A, E297K
16 894	S.-Y. Rhee	200	200	NA	NA	K20R, V35I, E36EK, M41L, K43N, D67N, I135V, G190E, T200A, L210W, T215Y, K219N, A272P, K277R
63 800	S.-Y. Rhee	200	200	NA	NA	S48T, D67N, T69DN, K70GR, L74V, K101KR, K104KR, D121Y, K122E, D123E, E169D, D177E, M184V, G190E, T200A, Q207K, F214L, K219EQ, K275KR, V276VI, P294T, E297KR
55 467	S.-Y. Rhee	200	200	NA	NA	D67G, S68G, L74V, Y115F, K122Q, D123E, I135T, I142V, D177E, M184V, V189I, G190E, T200A, T215F, K219E, V245E, E297R
127 280	G. L. Melikian	200	200	NA	NA	K46KIM, K65R, V75L, V90VI, K102KR, K103KR, Y115F, D123E, E169D, D177E, V179VI, G190E, T200A, R211K
33 143	W. Huang	200	200	NA	NA	K20R, M41L, K43Q, E44D, V60I, D67N, L74V, K102R, V118I, K122E, D123N, I135 V, S162Y, I178F, G190E, G196R, Q197K, T200A, Q207N, L210W, R211K, T215Y, K219N, E224N, A272P, T286A, E297K
91 738	J. D. Baxter	200	181	NA	NA	M41L, D67N, R72RG, D86DN, K122E, F160FL, K166R, D177E, V179G, G190E, T200A, Q207E, H208HD, L210W, T215Y, D218DE, K219N, K220KR, L228H

NVP, nevirapine; EFV, efavirenz; ETR, etravirine; RPV, rilpivirine; NA, not available.

^aThe creation of the cloned site-directed mutant 21176G190^E→G is described in the Methods section. The NRTI susceptibility results indicated slightly higher levels of decreased susceptibility than the 21 176 clone containing G190E, suggesting that the dramatic reductions in NNRTI resistance were not a result of decreased replication.

^bG190E is shown in bold and is underlined. Mutations in bold include L74V, which has been reported to partially restore the impaired replication capacity of viruses containing G190E,^21,22 and the accessory NNRTI-resistance mutation E138A.