Table 3.
Isolatea | Reference | Fold resistance |
Mutationsb | |||
---|---|---|---|---|---|---|
NVP | EFV | ETR | RPV | |||
21 176 | Stanford University | 200 | 200 | 200 | 200 | V60I, D67G, S68G, K70R, L74V, I135V, E138A, K173E, I178M, G190E, R211A, K219N, V245E, E248D, D250E, A272P, T286A |
21176G190E→G | 0.7 | 1.2 | 2.3 | 2.0 | V60I, D67G, S68G, K70R, L74V, I135V, E138A, K173E, I178M, R211A, K219N, V245E, E248D, D250E, A272P, T286A | |
24 746 | Stanford University | 200 | 200 | 94 | 28 | K20R, D67N, K70R, K122E, I135V, E138A, S162C, M184MV, G190E, T200A, F214M, T215F, K219Q, L228H, V245E |
59 209 | Stanford University | 200 | 200 | 110 | 7.3 | V35L, D67N, K70R, K101R, K103R, D177E, I178M, G190E, G196E, T200A, E204N, R211K, T215F, K219E, L228H, V245E, A272P, T286A, E297K |
16 894 | S.-Y. Rhee | 200 | 200 | NA | NA | K20R, V35I, E36EK, M41L, K43N, D67N, I135V, G190E, T200A, L210W, T215Y, K219N, A272P, K277R |
63 800 | S.-Y. Rhee | 200 | 200 | NA | NA | S48T, D67N, T69DN, K70GR, L74V, K101KR, K104KR, D121Y, K122E, D123E, E169D, D177E, M184V, G190E, T200A, Q207K, F214L, K219EQ, K275KR, V276VI, P294T, E297KR |
55 467 | S.-Y. Rhee | 200 | 200 | NA | NA | D67G, S68G, L74V, Y115F, K122Q, D123E, I135T, I142V, D177E, M184V, V189I, G190E, T200A, T215F, K219E, V245E, E297R |
127 280 | G. L. Melikian | 200 | 200 | NA | NA | K46KIM, K65R, V75L, V90VI, K102KR, K103KR, Y115F, D123E, E169D, D177E, V179VI, G190E, T200A, R211K |
33 143 | W. Huang | 200 | 200 | NA | NA | K20R, M41L, K43Q, E44D, V60I, D67N, L74V, K102R, V118I, K122E, D123N, I135 V, S162Y, I178F, G190E, G196R, Q197K, T200A, Q207N, L210W, R211K, T215Y, K219N, E224N, A272P, T286A, E297K |
91 738 | J. D. Baxter | 200 | 181 | NA | NA | M41L, D67N, R72RG, D86DN, K122E, F160FL, K166R, D177E, V179G, G190E, T200A, Q207E, H208HD, L210W, T215Y, D218DE, K219N, K220KR, L228H |
NVP, nevirapine; EFV, efavirenz; ETR, etravirine; RPV, rilpivirine; NA, not available.
aThe creation of the cloned site-directed mutant 21176G190E→G is described in the Methods section. The NRTI susceptibility results indicated slightly higher levels of decreased susceptibility than the 21 176 clone containing G190E, suggesting that the dramatic reductions in NNRTI resistance were not a result of decreased replication.