Table 2. The visible or not visible protein spots at 45°C vs 35°C (control) treatment gels scanned by UNAX Powerlook 2100XL.
| Spot# | Accession NO. | ORF | Gene product | COG Functional classification | MW (kDa,Theor./ Exper.) | pI (Theor./Exper.) | Matched Peptides | Cov | Score | E-value | Visible or not visible protein spots Vol‰ (Mean± SD) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 648 | ZP_03273305 | ORF0278 | Pyridoxal-5'-phosphate-dependent protein beta subunit [Arthrospira maxima] | Amino acid transport and metabolism | 34.84/37.95 | 5.28/5.23 | 26 | 69% | 241 | 8.30E-19 | +0.99±0.031 |
| 331 | ZP_06381552 | ORF0785 | Ribose-5-phosphate isomerase A [Arthrospira platensis] | Carbohydrate transport and metabolism | 25.13/28.28 | 4.96/5.04 | 10 | 46% | 105 | 3.30E-05 | + 0.50±0.045 |
| 1120 | ZP_06381999 | ORF0376 | Transketolase domain protein [Arthrospira platensis] | Carbohydrate transport and metabolism | 69.60/64.11 | 5.19/5.52 | 41 | 64% | 297 | 2.10E-24 | + 0.36±0.085 |
| 129 | ZP_03273475 | ORF1368 | Redoxin domain protein [Arthrospira maxima] | Post-translational modification, protein turnover, chaperones | 19.78/20.11 | 4.88/5.03 | 10 | 49% | 119 | 1.30E-06 | -0.75±0.037 |
| 224 | ZP_03275641 | ORF0073 | Ribosomal protein L6 [Arthrospira maxima] | Translation, ribosomal structure and biogenesis | 19.69/25.13 | 10.25/5.63 | 11 | 40% | 104 | 4.20E-05 | +0.60±0.025 |
| 433 | ZP_06380559 | ORF1074 | Elongation factor TS [Arthrospira platensis] | Translation, ribosomal structure and biogenesis | 24.63/31.08 | 5.74/5.90 | 21 | 77% | 155 | 3.30E-10 | +0.39±0.019 |
| 283 | ZP_03271593 | ORF4662 | Sigma54 modulation protein/ribosomal protein S30EA[Arthrospira maxima] | Translation, ribosomal structure and biogenesis | 23.97/29.22 | 7.03/6.22 | 9 | 58% | 94 | 4.00E-04 | -0.96±0.068 |
| 377 | ZP_06380559 | ORF1074 | Elongation factor TS [Arthrospira platensis] | Translation, ribosomal structure and biogenesis | 24.63/32.19 | 5.74/6.13 | 7 | 53% | 75 | 3.40E-02 | -0.83±0.076 |
| 118 | ZP_03271568 | ORF4634 | Phycocyanin, alpha subunit [Arthrospira maxima] | Energy production and conversion | 17.70/18.27 | 5.82/6.00 | 11 | 74% | 120 | 1.00E-06 | +1.67±0.031 |
| 143 | ZP_03271568 | ORF4634 | Phycocyanin, alpha subunit [Arthrospira maxima] | Energy production and conversion | 17.70/19.60 | 5.82/6.05 | 14 | 86% | 114 | 4.20E-06 | +0.35±0.043 |
| 70 | / | Phycocyanin, alpha subunit [Arthrospira maxima] | Energy production and conversion | 17.70/16.05 | 5.82/4.60 | 10 | 74% | 98 | 1.70E-05 | +0.44±0.055 | |
| 517 | ABB84420 | ORF4632 | Cpci [Arthrospira platensis] | Energy production and conversion | 32.79/33.33 | 8.33/5.68 | 17 | 51% | 127 | 2.10E-07 | +0.46±0.026 |
| 47 | ZP_03271327 | ORF4103 | Allophycocyanin, beta subunit [Arthrospira maxima] | Energy production and conversion | 17.43/14.81 | 6.26/5.74 | 14 | 84% | 165 | 3.30E-11 | -0.43±0.080 |
| 185 | ZP_06383806 | ORF0728 | Hypothetical protein aplap_19246 [Arthrospira platensis] | General function prediction only | 22.95/23.52 | 4.99/5.22 | 23 | 70% | 166 | 2.60E-11 | +0.46±0.045 |
| 886 | ZP_06384147 | ORF2097 | Von Willebrand factor, type A [Arthrospira platensis] | General function prediction only | 37.19/43.74 | 5.81/5.77 | 19 | 57% | 167 | 2.10E-11 | +0.86±0.023 |
| 934 | ZP_06381540 | ORF1251 | Hypothetical protein aplap_07632 [Arthrospira platensis] | General function prediction only | 67.41/65.34 | 5.66/6.21 | 19 | 42% | 166 | 2.60E-11 | -1.63±0.019 |
| 158 | ZP_06383329 | ORF4728 | Hypothetical protein aplap_16770 [Arthrospira platensis] | Function unknown | 19.34/21.31 | 5.30/5.83 | 9 | 59% | 120 | 1.00E-06 | +0.35±0.036 |
| 78 | ZP_06384870 | ORF4030 | Hypothetical protein aplap_24737 [Arthrospira platensis] | Function unknown | 17.32/16.31 | 5.96/6.14 | 19 | 86% | 254 | 4.10E-20 | -0.39±0.098 |
| 98 | ZP_06384870 | ORF4030 | Hypothetical protein aplap_24737 [Arthrospira platensis] | Function unknown | 17.32/17.19 | 5.96/6.50 | 18 | 82% | 214 | 4.10E-16 | -0.53±0.022 |
| 339 | ZP_06384062 | ORF0361 | Peptidase S8 and S53 subtilisin kexin sedolisin [Arthrospira platensis] | Function unknown | 44.24/31.22 | 4.59/4.65 | 16 | 44% | 200 | 1.00E-14 | -0.77±0.054 |
Note: (1) In “Visible or not visible protein spots” column, “+”indicates the visible protein spots in the 45°C treatment gels, while not visible in the 35°C control gels. “-” indicates the visible protein spots in the 35°C control gels, while not visible in the 45°C treatment gels.
(2) The relative abundance (Vol‰, Mean± SD) of “visible” protein calculated by the ratio of volume of each protein spot to volume of total protein spots in the whole gel.