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. 2013 Dec 12;8(12):e83423. doi: 10.1371/journal.pone.0083423

Table 1. Primers used for caspase-3A gene cloning and expression analysis.

Primer name Nucleotide sequence (5′-3′) Application
Upm (long) CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT RACE PCR
Upm (short) CTAATACGACTCACTATAGGGC Universal primers mix
Csp3F TGYATCATCATYAACAACAAGAA Conserved region cloning
Csp3R CATDGASACRATGCADGGGAT
Csp3A-5-1 AGCTGTGCTGTTTACAG 5′ RACE PCR
Csp3A-5-2 CCTAGCATCAAAGACTGGCTG
Csp3A-5-3 AAAGTCGAGTGCCACTTTATGG
Csp3A-3 CGGCTCTTGGTTCATTCAGTCCCTTTGT 3′ RACE PCR
Csp3-E ATAGAATTCATGAACGGAGACTGCGTG Recombinant expression
Csp3-X GAGCTCGAGTCAAGCAGTGAAGTACATCT
Csp3RTF GGTTCATTCAGTCCCTTTG Real-time PCR
Csp3RTR TACATCTCTTTGGTGAGCAT
β-actinF TTCTTGGGTATGGAGTCTTG Real-time PCR
β-actinR GTATTTACGCTCAGGTGGG