Table 1. Primers used for caspase-3A gene cloning and expression analysis.
Primer name | Nucleotide sequence (5′-3′) | Application |
Upm (long) | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | RACE PCR |
Upm (short) | CTAATACGACTCACTATAGGGC | Universal primers mix |
Csp3F | TGYATCATCATYAACAACAAGAA | Conserved region cloning |
Csp3R | CATDGASACRATGCADGGGAT | |
Csp3A-5-1 | AGCTGTGCTGTTTACAG | 5′ RACE PCR |
Csp3A-5-2 | CCTAGCATCAAAGACTGGCTG | |
Csp3A-5-3 | AAAGTCGAGTGCCACTTTATGG | |
Csp3A-3 | CGGCTCTTGGTTCATTCAGTCCCTTTGT | 3′ RACE PCR |
Csp3-E | ATAGAATTCATGAACGGAGACTGCGTG | Recombinant expression |
Csp3-X | GAGCTCGAGTCAAGCAGTGAAGTACATCT | |
Csp3RTF | GGTTCATTCAGTCCCTTTG | Real-time PCR |
Csp3RTR | TACATCTCTTTGGTGAGCAT | |
β-actinF | TTCTTGGGTATGGAGTCTTG | Real-time PCR |
β-actinR | GTATTTACGCTCAGGTGGG |