Figure 1.

(A and B) Rat myocardial apoptosis, analyzed using flow cytometry; (C) rat myocardial cell viability, assessed using the MTT method and (D and E) western blot analysis of cleaved caspase-3 and cleaved caspase-9 protein expression levels following 0, 24, 48 and 72 h of serum deprivation in rat myocardial cells. (F) MTT analysis of rat myocardial cell viability following the administration of alpinetin at various concentrations (40, 80 and 120 mg/ml) at the same time as serum deprivation, following incubation for 48 h. Data shown are representative results from at least three independent experiments. ^*P<0.05, versus control cells.