Figure 2.

(A and B) Expression levels of δ, κ and μ receptors in rat myocardial cells, as analyzed with western blotting 48 h subsequent to the serum deprivation of rat myocardial cells with simultaneous administration of 120 mg/ml alpinetin. ^*P<0.05, versus control cells. (C) MTT assay results of rat myocardial cell viability and (D and E) flow cytometry analysis of rat myocardial apoptosis and cell cycle following administration of the δ receptor antagonist naltrindole (10 μM) at the same time as alpinetin administration and serum deprivation. Data shown are the representative results from at least three independent experiments. ^*P<0.05, versus control group; ^**P<0.05, versus model group; ^#P<0.05, versus model + alpinetin group.