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. 2013 Dec 3;8(12):e81935. doi: 10.1371/journal.pone.0081935

Figure 2. Line-Weaver Burk plot of the stimulation of glucose induced NO synthesis in mice liver preparation.

Figure 2

The mice liver membrane in HEPES buffer, pH 7.4, was incubated in the presence and absence of 0.02M glucose with different amounts of l- arginine and 2.0mM CaCl2 in a total volume of 1.0ml. After incubation for 30 min at 37°C the synthesis of NO was determined by methemoglobin method as described in the Materials and Methods.

Line A represents the synthesis of NO in the absence of added glucose to the reaction mixture with different concentrations of l-arginine. Line B represents the synthesis of NO in the reaction mixture in the presence of 0.02M glucose and with different amounts of l-arginine.