Figure 3. Time course of glucose uptake by the GLS in the presence and absence of NAME.

The grated mice liver suspension (GLS) in HEPES buffer pH (7.4), was prepared as described in the Materials and Methods. Typically, 30-40 mg of GLS in the HEPES buffer, pH 7.4, was incubated in the presence of 0.02M nonmetabolizable 2-deoxy-D- glucose with 1µl of the ¹⁴C-labelled sugar as described in the Materials and Methods for different times as indicated. In parallel experiments, 0.1mM NAME was added to the identical reaction mixture and glucose uptake in both cases was determined.

Results are mean ± S.D. of 5 experiments each in triplicate using GLS preparation from 5 different animals.

Solid circles (●) represent the uptake of glucose by GLS in the presence of 0.02M glucose and solid squares (■) represent the uptake of glucose by GLS in the presence of both glucose and NAME in the reaction mixture respectively.