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. 2013 Dec 3;8(12):e81935. doi: 10.1371/journal.pone.0081935

Figure 5. Synthesis of Glut-4 in GLS incubated either with different amounts of glucose or with NO.

Figure 5

Typically 30 to 40 mg of GLS in HEPES buffer, pH 7.4, was incubated with different concentrations of glucose (0-0.05M) as shown or in the presence of 2.5nmol NO solution in 0.9% NaCl or in the presence of both 0.02M glucose and 2.5nmol NO solution for 30 min at 37°C. In parallel experiments, 0.1mM NAME was added to the reaction mixture containing 0.02M glucose. After incubation for 30 min at 37°C, the amounts of Glut-4 produced in the reaction mixture were determined by ELISA using Glut-4 antibody. The amount of Glut-4 produced in the reaction mixture was determined by expressing the increase of the arbitrary optical densities at 405 nm.

▓ - Glut-4 synthesis in the presence of varying amounts of glucose; ≡ - Glut-4 synthesis in the presence of both glucose and NO; □- synthesis of Glut-4 in the presence of only NO; ■- Glut-4 synthesis in the presence of glucose and NAME.

Results shown are mean ± S.D. of the optical densities obtained from 6 experiments by using 6 different animals.