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HMGN2 protein induces apoptosis in Tca8113 cells. (A and B) Quantitative analysis of cell apoptosis measured by Annexin V and PI double staining. (C) Examination of apoptosis by Hoechst assay. (D) Effect of HMGN2 treatment on the expression of GAPDH, p53, Bax, Bcl-2 and caspase-3. Data are presented as mean ± SEM for triplicate analyses. *P<0.05 and **P<0.01, vs. 0 μg/ml control. HMGN2, high mobility group nucleosomal binding domain 2.
