Figure 3.

(A) Hepatocellular carcinoma (HepG2) cells were cultured with varying concentrations of VX680 (25 and 50 μmol/l) for 24 h. All cells were collected and analyzed by western blot analysis with an anti-Aurora A antibody. (B) HepG2 cells were treated with 3.125 μmol/l VX680, 0.5 μg/ml cisplatin or a combination of the two for 72 h. Cell lysates were collected and analyzed by western blot analysis with anti-p53, anti-Bcl-2 and anti-β-actin antibodies. The protein level for each group was compared with that of the control group.