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. 2013 Oct 29;7(1):101–106. doi: 10.3892/ol.2013.1645

Figure 2.

Figure 2

Effect of AEG-1 knockdown on the proliferation and apoptosis in HepG2 cells. (A) The proliferation of the AEG-1 knockdown and control cells was detected by MTT assay (*P<0.05). (B and C) FACS cell cycle analysis of AEG-1 knockdown cells and vector control. (D and E) Colony formation assay of the HepG2-shAEG-1 and HepG2-vector cells (*P<0.05). (F and G) Apoptosis analysis of the HepG2-shAEG-1 and HepG2-vector cells was detected by flow cytometry subsequent to staining the cells with Annexin V/PE (*P<0.05). Error bars represent SD, n=3 experiments. AEG-1, astrocyte-elevated gene-1; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; FACS, fluorescence-activated cell sorting; PE, phycoerythrin.